The use of different LEDs wavelength and light intensities for in vitro proliferation of cherry rootstock: influence on photosynthesis and photomorphogenesis

Abstract Plant growth and development are strongly influenced by light quality conditions in their environment. In this study, vitro shoot proliferation, photosynthetic pigments, leaf anatomy photosystem II photochemistry of Gisela 6 cherry rootstock ( Prunus cerasus x canescens ) were investigated. The culture medium used was the MS (Murashige Skoog) containing 4.4 μΜ 6-benzyladenine (BA), 0.05 α-naphthalene acetic acid (NAA) 0.3 μM gibberellic (GA 3 (30 days, 16 h photoperiod, 22 ± 2 °C). Initially, two different wavelength absorption spectra: 400–700 nm provided white fluorescent lamps (mainly blue–green, BG at equal ratio 1:1; WFL-40) and, 430–690 Light Emitting Diodes (LED) types, differing spectral multiple emitting wavelengths [LED2-40 (blue–red, BR) LED3-40 (blue–green–yellow–orange–red, BGYOR)] studied, under 40 μmol m −2 s −1 intensity. Following that, three LED intensities-20 (LED1-20), (LED2-40), 120 (LED4-120) investigated BR spectrum. WFL-40 exhibited non-significant differences with LED2-40 (shoot number 4.25, proliferation rate 4.30, height 0.91 cm). displayed higher fresh dry biomass, better PSII functionality, increased Chl a/b, b carotenoids content as well decreased stressed explants. Leaf similar. LED4-120 presented highest explants, being severely affected, leaves had a reduced mesophyll cell density. LED4-120, proportion absorbed energy (Φ whereas quantum yield non-regulated loss NO could not be compensated photoprotective dissipation NPQ ). Therefore, is proposed most beneficial for growing plantlets vitro.